Using the method of molecular genetic typing to determine variants of the weak antigen D in the diagnosis of Rh-affiliation

Background. Determination of Rh-affiliation is mandatory for donors and recipients, surgical patients, pregnant women, etc. There are variants the D antigen that difficult to identify by serological methods, example, a weak antigen.
 Aim. using genotyping in cases, when use methods does not allow obtaining reliable result.
 Material methods. We studied blood samples from (n=18), women (n=17) patients with hematological diseases (n=15): 22 men 28 median age 36 years (25 54 years). Serologically, was determined gel technology ID-cards an indirect antiglobulin test anti-D-IgG reagent. Weak were diagnosed phenotype polymerase chain reaction allele-specific primers. For significance differences frequency types D, nonparametric statistical method two-tailed Fisher's exact used. Differences considered statistically significant at p 0.05.
 Results. The made it possible detect presence 41 samples. Its specificity represented following types: 1; 1.1; 2; 3. Other [4; 4.0; 4.1; 4.2 (DAR); 5; 11; 14; 15; 17] absent. study erythrocyte antigens Rhesus system revealed predominance Ccee people antigen. Significant this revealed.
 Conclusion. molecular genetic typing determine accurately subjects.